Review



lambda zap express vector  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies lambda zap express vector
    Lambda Zap Express Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/pmc02920863-45-6-10
    Average 90 stars, based on 1 article reviews
    lambda zap express vector - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Autoantibodies Targeting a Collecting Duct–Specific Water Channel in Tubulointerstitial Nephritis
    Article Snippet: A Poly A Sepharose Purist (Ambion Inc., Austin, TX) was used to extract the mRNA fraction. .. Isolated mRNA was converted to cDNA and cloned into a Zap Express Vector (Stratagene, La Jolla, CA) according to the instructions from the supplier. ..

    Article Title: Nucleic acid molecules and polypeptides for immune modulation
    Article Snippet: First strand cDNA is synthesized from RNA prepared from a tissue of interest and PCR is performed with an initial five cycle step, for example, at 37° C. for 60 s, followed by 25 cycles at 50° C. for 60 s (denaturation at 95° C. for 30 s and extension at 72° C. for 90 s) in order to amplify a Yatapoxvirus-related gp38 cDNA fragment that can be subsequently subcloned into Bluescript II KS (Stratagene). .. Construction of a cDNA library using poly A RNA isolated from the selected tissue is performed using Stratagene ZAP Express Vector according to the directions of the manufacturer. ..

    Article Title: Autoantibodies Targeting a Collecting Duct–Specific Water Channel in Tubulointerstitial Nephritis
    Article Snippet: A Poly A Sepharose Purist (Ambion Inc., Austin, TX) was used to extract the mRNA fraction. .. Isolated mRNA was converted to cDNA and cloned into a Zap Express Vector (Stratagene, La Jolla, CA) according to the instructions from the supplier. ..

    Clone Assay:

    Article Title: Autoantibodies Targeting a Collecting Duct–Specific Water Channel in Tubulointerstitial Nephritis
    Article Snippet: A Poly A Sepharose Purist (Ambion Inc., Austin, TX) was used to extract the mRNA fraction. .. Isolated mRNA was converted to cDNA and cloned into a Zap Express Vector (Stratagene, La Jolla, CA) according to the instructions from the supplier. ..

    Article Title: Tissue-Specific Expressed Sequence Tags from the Black Tiger Shrimp Penaeus monodon.
    Article Snippet: Expressed sequence tag data were generated from complementary DNA libraries created from cephalothorax, eyestalk, and pleopod tissue of the black tiger shrimp (Penaeus monodon).. Significant database matches were found for 48 of 83 nuclear genes sequenced from the cephalothorax library, 22 of 55 nuclear genes from the eyestalk library, and 6 of 13 nuclear genes from the pleopod library.. The putative identities of these genes reflected the expected tissue specificity.

    Article Title: Autoantibodies Targeting a Collecting Duct–Specific Water Channel in Tubulointerstitial Nephritis
    Article Snippet: A Poly A Sepharose Purist (Ambion Inc., Austin, TX) was used to extract the mRNA fraction. .. Isolated mRNA was converted to cDNA and cloned into a Zap Express Vector (Stratagene, La Jolla, CA) according to the instructions from the supplier. ..

    cDNA Library Assay:

    Article Title: Nucleic acid molecules and polypeptides for immune modulation
    Article Snippet: First strand cDNA is synthesized from RNA prepared from a tissue of interest and PCR is performed with an initial five cycle step, for example, at 37° C. for 60 s, followed by 25 cycles at 50° C. for 60 s (denaturation at 95° C. for 30 s and extension at 72° C. for 90 s) in order to amplify a Yatapoxvirus-related gp38 cDNA fragment that can be subsequently subcloned into Bluescript II KS (Stratagene). .. Construction of a cDNA library using poly A RNA isolated from the selected tissue is performed using Stratagene ZAP Express Vector according to the directions of the manufacturer. ..

    Article Title: Identification of a Novel Human E-Cadherin Splice Variant and Assessment of Its Effects Upon EMT-Related Events.
    Article Snippet: This article is protected by copyright.. All rights reserved 1

    Purification:

    Article Title: Tissue-Specific Expressed Sequence Tags from the Black Tiger Shrimp Penaeus monodon.
    Article Snippet: Expressed sequence tag data were generated from complementary DNA libraries created from cephalothorax, eyestalk, and pleopod tissue of the black tiger shrimp (Penaeus monodon).. Significant database matches were found for 48 of 83 nuclear genes sequenced from the cephalothorax library, 22 of 55 nuclear genes from the eyestalk library, and 6 of 13 nuclear genes from the pleopod library.. The putative identities of these genes reflected the expected tissue specificity.

    Plasmid Preparation:

    Article Title: Tissue-Specific Expressed Sequence Tags from the Black Tiger Shrimp Penaeus monodon.
    Article Snippet: Expressed sequence tag data were generated from complementary DNA libraries created from cephalothorax, eyestalk, and pleopod tissue of the black tiger shrimp (Penaeus monodon).. Significant database matches were found for 48 of 83 nuclear genes sequenced from the cephalothorax library, 22 of 55 nuclear genes from the eyestalk library, and 6 of 13 nuclear genes from the pleopod library.. The putative identities of these genes reflected the expected tissue specificity.

    Article Title: Human sarcoma-associated antigens
    Article Snippet: Poly(A)+ RNA from normal testis was purchased from CLONTECH. .. Separate cDNA libraries were constructed for each of these in the ZAP Express vector (Stratagene, La Jolla, Calif.) according to the manufacturer's instructions using 5 μg polyA+ mRNA. ..

    Article Title: Recombinant antigens for immunodiagnosis of cystic echinococcosis
    Article Snippet: .. The cDNAs were ligated into the ZAP Express vector (Stratagene) in a final volume of 5 ml containing 1 ml of the ZAP Express vector (1 mg/ml), 2 ml of cDNA (about 200 ng), 0.5 ml of 10 × ligase buffer, 0.5 ml of 10 mM rATP (pH 7.5), 0.5 ml of water and 0.5 ml of T4 DNA ligase (4 U/ml) (all supplied by Stratagene). ..

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    cDNA Synthesis:

    Article Title: Tissue-Specific Expressed Sequence Tags from the Black Tiger Shrimp Penaeus monodon.
    Article Snippet: Expressed sequence tag data were generated from complementary DNA libraries created from cephalothorax, eyestalk, and pleopod tissue of the black tiger shrimp (Penaeus monodon).. Significant database matches were found for 48 of 83 nuclear genes sequenced from the cephalothorax library, 22 of 55 nuclear genes from the eyestalk library, and 6 of 13 nuclear genes from the pleopod library.. The putative identities of these genes reflected the expected tissue specificity.

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    Construct:

    Article Title: Human sarcoma-associated antigens
    Article Snippet: Poly(A)+ RNA from normal testis was purchased from CLONTECH. .. Separate cDNA libraries were constructed for each of these in the ZAP Express vector (Stratagene, La Jolla, Calif.) according to the manufacturer's instructions using 5 μg polyA+ mRNA. ..

    Article Title: Identification of a Novel Human E-Cadherin Splice Variant and Assessment of Its Effects Upon EMT-Related Events.
    Article Snippet: This article is protected by copyright.. All rights reserved 1

    Expressing:

    Article Title: Identification of a Novel Human E-Cadherin Splice Variant and Assessment of Its Effects Upon EMT-Related Events.
    Article Snippet: This article is protected by copyright.. All rights reserved 1

    Inhibition:

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    Modification:

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    Clonogenic Cell Survival Assay:

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    Library Screening:

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..

    Produced:

    Article Title: Protein fragment complementation assays for the detection of biological or drug interactions
    Article Snippet: .. Further controls will be performed for inhibition of protein association with wortmannin and rapamycin. e) Modification of the Eukaryotic Survival Assay for Library Screening An important part of the work required in creating a library for use in eukaryotic cells will have been accomplished already, as the EcoRI/XhoI directional cDNA produced by the Stratagene “cDNA Synthesis Kit” can directly be inserted directionally into the Stratagene Zap Express vector. ..



    Similar Products

    90
    Agilent technologies lambda zap express vector
    Lambda Zap Express Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/pmc02920863-45-6-10
    Average 90 stars, based on 1 article reviews
    lambda zap express vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies lambda-zap express vector
    Lambda Zap Express Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/pmc02762978-256-12-15
    Average 90 stars, based on 1 article reviews
    lambda-zap express vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Millipore λ zap-cmv-apoptin expressing vector
    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and <t>apoptin</t> transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ <t>ZAP-CMV</t> vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.
    λ Zap Cmv Apoptin Expressing Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/%CE%BB+zap+cmv+apoptin+expressing+vector/pmc03838365-204-4-16
    Average 90 stars, based on 1 article reviews
    λ zap-cmv-apoptin expressing vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies zap express vector
    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and <t>apoptin</t> transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ <t>ZAP-CMV</t> vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.
    Zap Express Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/pmc00411165-108-6-9
    Average 90 stars, based on 1 article reviews
    zap express vector - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies zap express vector kit
    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and <t>apoptin</t> transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ <t>ZAP-CMV</t> vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.
    Zap Express Vector Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/us11053514-784-11-16
    Average 90 stars, based on 1 article reviews
    zap express vector kit - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    93
    Addgene inc zap expression vector
    The involvement of <t>ZAP</t> in the regulation of HTLV-1 production. a HeLa cells were transfected with a Tax expression vector in the presence of increasing amounts of a ZAP expression vector. Changes in the level of tax transcripts (top) were measured by RT-qPCR, in addition to those of hZAP (bottom). *p < 0.0001. <t>b</t> <t>JEX22</t> cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with PMA/ionomycin was performed for 4 h before collecting the supernatant and cells for analysis. c , d JEX22 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated, as indicated previously in this figure, before analysis. HTLV-1 RNAs ( c ) or p19 protein ( d ) was was measured (top panel) upon knock-down of endogenous ZAP (bottom panel). e J1.1 cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with TNFα was performed for 4 h before collecting the supernatant and the cells for analysis. f J1.1 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated as indicated before analysis. No significant changes were observed in p24 protein levels in the supernatant (top panel) upon knock-down of endogenous ZAP (bottom panel). *p < 0.05; **p ≤ 0.001; ***p < 0.0001
    Zap Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zap+express+vector/pcDNA4+huZAP(L)+(Plasmid+%2345907)/pmc06915898-181-15-18
    Average 93 stars, based on 1 article reviews
    zap expression vector - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Expressing, Plasmid Preparation, Recombinant, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Purification, Positive Control, Negative Control

    Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Immunostaining, Plasmid Preparation

    BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation

    BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation, Construct, Staining, Immunostaining, Fluorescence, Microscopy

    ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques:

    ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: TUNEL Assay, Activity Assay, Infection, Plasmid Preparation

    The involvement of ZAP in the regulation of HTLV-1 production. a HeLa cells were transfected with a Tax expression vector in the presence of increasing amounts of a ZAP expression vector. Changes in the level of tax transcripts (top) were measured by RT-qPCR, in addition to those of hZAP (bottom). *p < 0.0001. b JEX22 cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with PMA/ionomycin was performed for 4 h before collecting the supernatant and cells for analysis. c , d JEX22 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated, as indicated previously in this figure, before analysis. HTLV-1 RNAs ( c ) or p19 protein ( d ) was was measured (top panel) upon knock-down of endogenous ZAP (bottom panel). e J1.1 cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with TNFα was performed for 4 h before collecting the supernatant and the cells for analysis. f J1.1 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated as indicated before analysis. No significant changes were observed in p24 protein levels in the supernatant (top panel) upon knock-down of endogenous ZAP (bottom panel). *p < 0.05; **p ≤ 0.001; ***p < 0.0001

    Journal: Retrovirology

    Article Title: HTLV-1 contains a high CG dinucleotide content and is susceptible to the host antiviral protein ZAP

    doi: 10.1186/s12977-019-0500-3

    Figure Lengend Snippet: The involvement of ZAP in the regulation of HTLV-1 production. a HeLa cells were transfected with a Tax expression vector in the presence of increasing amounts of a ZAP expression vector. Changes in the level of tax transcripts (top) were measured by RT-qPCR, in addition to those of hZAP (bottom). *p < 0.0001. b JEX22 cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with PMA/ionomycin was performed for 4 h before collecting the supernatant and cells for analysis. c , d JEX22 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated, as indicated previously in this figure, before analysis. HTLV-1 RNAs ( c ) or p19 protein ( d ) was was measured (top panel) upon knock-down of endogenous ZAP (bottom panel). e J1.1 cells were transfected with increasing amounts of a human ZAP expression vector and cultured for 24 h. Stimulation with TNFα was performed for 4 h before collecting the supernatant and the cells for analysis. f J1.1 cells were transfected with the indicated siRNAs and cultured for 24 h. They were then stimulated as indicated before analysis. No significant changes were observed in p24 protein levels in the supernatant (top panel) upon knock-down of endogenous ZAP (bottom panel). *p < 0.05; **p ≤ 0.001; ***p < 0.0001

    Article Snippet: For ZAP over-expression experiments, JEX22 or J1.1 cells were electroporated with increasing amounts of a ZAP expression vector (Addgene number 45907).

    Techniques: Transfection, Expressing, Plasmid Preparation, Quantitative RT-PCR, Cell Culture, Knockdown